- Does higher RF mean more polar?
- Do RF values have units?
- Can two compounds have the same Rf value?
- What does a large Rf value indicate?
- What are the RF values of pigments?
- Is RF dependent on polarity?
- Do more polar solvents elute faster?
- What do Rf values indicate?
- Why do compounds have different RF values in different solvents?
- How would Rf values change if a more polar solvent were used?
- Can an RF be greater than 1?
- Would the RF value change if the solvent was changed?
- What are good RF values?
- Do all compounds have unique RF values?
- Why do we use RF values?
- Why is RF always less than 1?
- What factors affect RF values in paper chromatography?
Does higher RF mean more polar?
In general, the adsorptivity of compounds increases with increased polarity (i.e.
the more polar the compound then the stronger it binds to the adsorbent).
Non-polar compounds move up the plate most rapidly (higher Rf value), whereas polar substances travel up the TLC plate slowly or not at all (lower Rf value)..
Do RF values have units?
Rf values do not have units since it is a ration of distances. Because mixture solvents are often applied Rf values are usually written as the following examples: … Note that mixture compounds with larger proportions are placed first in the mixture sequence.
Can two compounds have the same Rf value?
The Rf can provide corroborative evidence as to the identity of a compound. … If two substances have the same Rf value, they are likely (but not necessarily) the same compound. If they have different Rf values, they are definitely different compounds.
What does a large Rf value indicate?
Rf = distance traveled by substance/distance traveled by solvent front. A high Rf (Ie 0.92) would refer to a substance that is very non-polar. Ie that substance moved a 92% of the entire distance the solvent traveled. A low Rf value (0.10) would refer to a substance that is very polar.
What are the RF values of pigments?
The Rf value varies depending on the solvent used, but the general order of the pigments (from the highest to the lowest Rf value) usually remains the same, because the nonpolar compounds move further than the polar compounds….Explanation.PigmentRf valueChlorophyll b0.42Anthocyanins0.32-0.62Xanthophylls0.15-0.352 more rows•Jun 21, 2019
Is RF dependent on polarity?
Rf is dependent on polarity. Polar compounds will have lower Rf values than nonpolar compounds under the same developing conditions. The more polar the solvent, the higher the Rf values.
Do more polar solvents elute faster?
Note that the more polar the solvent, the faster compounds elute, regardless of the compounds polarity. … This will force compounds into the mobile phase, and result in faster elution/increased travel distance. It may also be helpful to remember that alumina and silica are much more polar than any organic solvent.
What do Rf values indicate?
The Rf values indicate how soluble the particular pigment is in the solvent by how high the pigment moves on the paper. Two pigments with the same Rf value are likely to be identical molecules. Small Rf values tend to indicate larger, less soluble pigments while the highly soluble pigments have an Rf value near to one.
Why do compounds have different RF values in different solvents?
The Rf value The larger an Rf of a compound, the larger the distance it travels on the TLC plate. When comparing two different compounds run under identical chromatography conditions, the compound with the larger Rf is less polar because it interacts less strongly with the polar adsorbent on the TLC plate.
How would Rf values change if a more polar solvent were used?
In general, how will Rf values change with increasing solvent polarity? Rf values increase with increasing solvent polarity as substance are more attracted to the solvent and therefore move with it.
Can an RF be greater than 1?
By definition, Rf values are always less than 1. An Rf value of 1 or too close to it means that the spot and the solvent front travel close together and is therefore unreliable. This happens when the eluting solvent is too polar for the sample.
Would the RF value change if the solvent was changed?
Based on the Rf values, xanthophylls are more soluble in the chromatography solvent. … If altering the composition of the solvent resulted in a change the solubility of the pigment in the solvent, the Rf value would also change.
What are good RF values?
The best Rf (retention or retardation factor) lies between 0.3 and 0.7. If you want the Rf of your TLC spot to be smaller, i.e., the spot to be lower down on the plate, you must decrease the eluent polarity.
Do all compounds have unique RF values?
Rf or Retention factor is a unique value for each compound under the same conditions. The Rf for a compound is a constant from one experiment to the next only if the chromatography conditions below are also constant: solvent system. adsorbent.
Why do we use RF values?
R f values can be used to identify unknown chemicals if they can be compared to a range of reference substances. The Rf value for a particular substance is always the same if the same solvent and stationary phase are used.
Why is RF always less than 1?
An Rf value will always be in the range 0 to 1; if the substance moves, it can only move in the direction of the solvent flow, and cannot move faster than the solvent. For example, if particular substance in an unknown mixture travels 2.5 cm and the solvent front travels 5.0 cm, the retardation factor would be 0.5.
What factors affect RF values in paper chromatography?
Rf values and reproducibility can be affected by a number of different factors such as layer thickness, moisture on the TLC plate, vessel saturation, temperature, depth of mobile phase, nature of the TLC plate, sample size, and solvent parameters. These effects normally cause an increase in Rf values.